The research was focused on the refining of the fiber bundles of hemp. The separation of the bundles into fiber cells was caused by cell wall degrading enzymes and ultrasonic treatment. Changes of the fiber structure were followed at molecular and microscopic levels by means of NIR FT Raman spectroscopy and Environmental Scanning Electron Microscopy (ESEM).
Buffer-panzym treatments of hemp fibers have a prominent effect in loosening of the fiber cells. The best of refining is achieved when the fiber bundles are treated with buffer-panzym solution in combination with ultrasonic.